Journal: bioRxiv

Article Title: Mitochondrial inner membrane remodeling as a driving force of organelle shaping

doi: 10.1101/2024.05.27.595707

Figure Lengend Snippet: Western Blots for validation of the siRNA pools used to knockdown OPA1 and ATP5ME. For transfection, 5.2x 10 5 HeLa cells were seeded into a 60mm culture dish with regular culture medium. Upon 72h, cells were harvested and subjected to Western Blotting using the respective antibodies. A) Knockdown of Opa1. B) Knockdown of ATPase subunit e. Beta-actin served as loading control.

Article Snippet: All knockdowns were verified by Western blotting using antibodies against Opa1 (67589, Cell Signaling Technology, USA), Mic10 (ab84969, Abcam, UK), Mic60 (10179-1-AP, Proteintech, USA) and ATPase subunit e (16483-1-AP, Proteintech, USA), respectively.

Techniques: Western Blot, Biomarker Discovery, Knockdown, Transfection, Control

Journal: International Journal of Molecular Sciences

Article Title: Chrono-Aerobic Exercise Optimizes Metabolic State in DB/DB Mice through CLOCK–Mitophagy–Apoptosis

doi: 10.3390/ijms23169308

Figure Lengend Snippet: Diabetes dramatically impairs mitochondrial quality, and exercise ameliorates excessive mitophagy caused by diabetes. ( A ) Wes TM of OPA1, Mfn1, Drp1, and Fis1 expression in livers from WT, DB, DM, and DE mice. ( B ) Statistical evaluation of OPA1 expression in livers. *** p < 0.001, n = 3. ( C ) Statistical evaluation of Mfn1 expression in livers, n = 3 to 4. ( D ) Statistical evaluation of Drp1 expression in livers, n = 3. ( E ) Statistical evaluation of Fis1 expression in livers. ** p < 0.01, n = 3. ( F ) Representative images of Parkin and LC3 in livers from WT, DB, DM, and DE mice ( n = 3). ( G ) Statistical evaluation of Parkin of ( F ), ** p < 0.01 and *** p < 0.001. ( H ) Statistical evaluation of LC3 of ( F ), *** p < 0.001. Scale bar: 50 μm.

Article Snippet: Polyclonal antibodies against OPA1 (sc-367890, Santa Cruz, 1:50), Mfn1 (ab57602, Abcam, 1:250), Drp1 (ab184248, Abcam, 1:250), Fis1 (10956-1-AP, Proteintech, 1:85), HSP90 (ab178854, Abcam, 1:250), β-Tubulin (AF7011, Proteintech, 1:50), and horseradish peroxidase-conjugated secondary antibodies were diluted 1:25 (Santa Cruz Biotechnology Inc., Dallas, TX, USA).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Chrono-Aerobic Exercise Optimizes Metabolic State in DB/DB Mice through CLOCK–Mitophagy–Apoptosis

doi: 10.3390/ijms23169308

Figure Lengend Snippet: The CLOCK expression is negatively correlated with Fis1 and OPA1 expression. ** p < 0.01 and *** p < 0.001.

Article Snippet: Polyclonal antibodies against OPA1 (sc-367890, Santa Cruz, 1:50), Mfn1 (ab57602, Abcam, 1:250), Drp1 (ab184248, Abcam, 1:250), Fis1 (10956-1-AP, Proteintech, 1:85), HSP90 (ab178854, Abcam, 1:250), β-Tubulin (AF7011, Proteintech, 1:50), and horseradish peroxidase-conjugated secondary antibodies were diluted 1:25 (Santa Cruz Biotechnology Inc., Dallas, TX, USA).

Techniques: Expressing